Nucleocytoplasmic shuttling of endocytic proteins

被引:86
作者
Vecchi, M
Polo, S
Poupon, V
van de Loo, JW
Benmerah, A
Di Fiore, PP
机构
[1] European Inst Oncol, Dept Expt Oncol, I-20141 Milan, Italy
[2] Fac Necker Enfants Malad, Inst Natl Sante & Rech Med E9925, F-75730 Paris, France
[3] Fdn Italiana Ric Canc, Inst Mol Oncol, Inst Format Metiers Paramedicaux, I-20139 Milan, Italy
[4] Univ Milan, Dipartimento Med Chirurg & Odontoiatria, I-20122 Milan, Italy
关键词
endocytosis; nucleocytoplasmic shuttling; eps15; CRM1; transcription;
D O I
10.1083/jcb.153.7.1511
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly Lymphoid myeloid leukemia (CALM), and alpha -adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic shuttling of endocytic proteins are apparently independent processes, since inhibition of endocytosis did not appreciably alter nuclear translocation of endocytic proteins, and blockade of nuclear export did not change the initial rate of endocytosis. In the nucleus, eps15 and CALM acted as positive modulators of transcription in a GAL4-based transactivation assay, thus raising the intriguing possibility that some endocytic proteins play a direct or indirect role in transcriptional regulation.
引用
收藏
页码:1511 / 1517
页数:7
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