Evaluation of restriction enzymes for standardizing pulsed-field gel electrophoresis protocol for rapid subtyping of Vibrio parahaemolyticus

被引：8

作者：

Wang, You-Wun ^{[1
]}

Chern, Lih-Ling

Cam, Phung Dac

Chiou, Chien-Shun ^{[1
,2
]}

机构：

[1] Ctr Dis Control, Cent Reg Lab, Ctr Res & Diagnost, Taichung 40855, Taiwan

[2] Chung Shan Med Univ, Inst Med, Taichung 40201, Taiwan

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 03期

关键词：

molecular typing; PulseNet; pulsed-field gel electrophoresis; Vibrio parahaemolyticus;

D O I：

10.1016/j.diagmicrobio.2008.01.024

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We evaluated the cost-effectiveness of the restriction enzymes with rare-cutting sites in the genome of Vibrio parahaemolyticus RIMD 2210633 for pulsed-field gel electrophoresis (PFGE) analysis. The evaluation indicated that PFGE with both NotI and SfiI was discriminatory, but NotI was more cost-effective. Based on the results of this study, we suggest using NotI and SfiI as the 1st and the 2nd restriction enzyme for standardizing the PulseNet PFGE protocol for molecular subtyping and global surveillance of V. parahaemolyticus. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：251 / 255

页数：5

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