Identification of mouse AAV capsid-specific CD8+ T cell epitopes

被引：76

作者：

Sabatino, DE

Mingozzi, F

Hui, DJ

Chen, HF

Colosi, P

Ertl, HCJ

High, KA ^{[1
]}

机构：

[1] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA

[2] Avigen Inc, Alameda, CA USA

[3] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA

[4] Howard Hughes Med Inst, Philadelphia, PA USA

来源：

MOLECULAR THERAPY | 2005年 / 12卷 / 06期

关键词：

adeno-associated virus; gene therapy; CD8(+) T cells; epitopes; AAV-2; AAV-8; AAV capsid;

D O I：

10.1016/j.ymthe.2005.09.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Adeno-associated virus has been developed for use as a gene transfer vector. To understand the impact of AAV capsid-specific CD8(+) T cells on AAV-mediated gene transfer, we identified CD8(+) T cell epitopes for AAV-2 and AAV-8 capsid in C57BL/6 (H-2(b) MHC haplotype) and BALB/c (H-2(d) MHC haplotype). mice. Mice of both the H-2(b) and the H-2(d) haplotypes recognized epitopes on AAV-2 and AAV-8 capsid. T cells from H-2 mice recognized an epitope that was conserved between AAV-2 and AAV-8 capsid. Cross-reactivity of AAV-specific CD8(+) T cells induced by different AAV serotypes may have important implications for gene transfer. Identification of these epitopes will facilitate studies of immune response to AAV capsid in mouse models.

引用

页码：1023 / 1033

页数：11

共 32 条

[1] Gene therapy restores vision in a canine model of childhood blindness [J].