Medium optimization for recombinant protein production by Bacillus subtilis

被引：14

作者：

Park, K ^{[1
]}

Reardon, KF ^{[1
]}

机构：

[1] COLORADO STATE UNIV,DEPT CHEM & BIORESOURCE ENGN,FT COLLINS,CO 80523

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 06期

关键词：

D O I：

10.1007/BF00130775

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Optimal concentrations of glucose, yeast extract and nutrient broth for the production of beta-lactamase by B. subtilis were predicted using a statistical experimental design and then tested. More yeast extract (13 vs. 1 g/L), less glucose (7.4 vs. 10 g/L), and less nutrient broth (12.6 vs. 15 g/L) were required to achieve high beta-lactamase activities (5700 U/L) instead of high cell growth rates (1.2 h(-1)).

引用

页码：737 / 740

页数：4

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