Cloning of cDNA encoding a novel isoform (type IV) of peptidylarginine deiminase from rat epidermis

被引：23

作者：

Yamakoshi, A ^{[1
]}

Ono, H ^{[1
]}

Nishijyo, T ^{[1
]}

Shiraiwa, M ^{[1
]}

Takahara, H ^{[1
]}

机构：

[1] Ibaraki Univ, Sch Agr, Dept Appl Biol Resource Sci, Ibaraki, Osaka 3000393, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1386卷 / 01期

关键词：

cDNA sequence; peptidylarginine deiminase type IV; (rat);

D O I：

10.1016/S0167-4838(98)00084-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a new clone that showed structural similarities with the rat peptidylarginine deiminase (PAD) types II and III. The full-length cDNA sequence of this novel PAD comprised 1998 bp encoding a sequence for 666 amino acid residues (M-r 74 467), a 3'-non-coding region of 115 bp and a 5'-non-coding region of 16 bp. The derived amino acid sequence of the PAD showed 51.1 and 54.0% identities with the sequences of types II and III, respectively. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assays of mRNAs from several tissues of rat indicated that the PAD message is highly expressed in the pancreas, spleen, and ovary and, less strongly expressed in the liver, lung, stomach, kidney, uterus, and dermis, and weakly expressed in the brain, heart and epidermis. Since this expression pattern was quite different from those of the previously reported PAD types I, II, and III, we designated this novel PAD as type IV. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：227 / 232

页数：6

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