Dissection of the cAMP induced cytosolic calcium response in Dictyostelium discoideum:: the role of cAMP receptor subtypes and G protein subunits

被引：12

作者：

Sonnemann, J ^{[1
]}

Aichem, A ^{[1
]}

Schlatterer, C ^{[1
]}

机构：

[1] Univ Konstanz, Fak Biol, D-78457 Constance, Germany

来源：

FEBS LETTERS | 1998年 / 436卷 / 02期

关键词：

development; signal transduction; G(alpha 2); G beta; fura2-dextran; Ca2+](i) imaging;

D O I：

10.1016/S0014-5793(98)01139-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cAMP signaling cascade leading to changes in [Ca2+](i) in Dictyostelium discoideum was analyzed using cell lines overexpressing single cAMP receptor subtypes (cAR1-cAR3) or lacking the G(alpha 2) or G(beta) Subunit of the G protein. Imaging of fura2-destran-loaded amoebae revealed cAMP-induced [Ca2+](i) changes characteristic for each receptor subtype activated. Cells expressing distinct subtypes sort to defined zones during multicellular development suggesting involvement of the specific [Ca2+](i) transients in patterning processes. Whereas generation of the [Ca2+](i) increase was G(alpha 2)-independent, only few cells devoid of Gp displayed a [Ca2+](i) change after stimulation indicating its participation in the regulation of the calcium homeostasis. (C) 1998 Federation of European Biochemical Societies.

引用

页码：271 / 276

页数：6

共 45 条

[1] A HIGH-AFFINITY PLASMA-MEMBRANE CA-2+-ATPASE IN DICTYOSTELIUM-DISCOIDEUM - ITS RELATION TO CAMP-INDUCED CA-2+ FLUXES [J].