Arabidopsis RecQl4A suppresses homologous recombination and modulates DNA damage responses

被引：51

作者：

Bagherieh-Najjar, MB ^{[1
]}

de Vries, OMH ^{[1
]}

Hille, J ^{[1
]}

Dijkwel, PP ^{[1
]}

机构：

[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, NL-9751 NN Haren, Netherlands

来源：

PLANT JOURNAL | 2005年 / 43卷 / 06期

关键词：

Arabidopsis; DNA repair; genome instability; DNA recombination; RecQ;

D O I：

10.1111/j.1365-313X.2005.02501.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The DNA damage response and DNA recombination are two interrelated mechanisms involved in maintaining the integrity of the genome, but in plants they are poorly understood. RecO is a family of genes with conserved roles in the regulation of DNA recombination in eukaryotes; there are seven members in Arabidopsis. Here we report on the functional analysis of the Arabidopsis RecQ14A gene. Ectopic expression of Arabidopsis RecQ14A in yeast RecQ-deficient cells suppressed their hypersensitivity to the DNA-damaging drug methyl methanesulfonate (MMS) and enhanced their rate of homologous recombination (HR). Analysis of three recQ14A mutant alleles revealed no obvious developmental defects or telomere deregulation in plants grown under standard growth conditions. Compared with wild-type Arabidopsis, the recQ14A mutant seedlings were found to be hypersensitive to UV light and MMS, and more resistant to mitomycin C. The average frequency of intrachromosomal HR in recQ14A mutant plants was increased 7.5-fold over that observed in wild-type plants. The data reveal roles for Arabidopsis RecQ14A in maintenance of genome stability by modulation of the DNA damage response and suppression of HR.

引用

页码：789 / 798

页数：10

共 61 条

[1]

[Anonymous], PLANT TISSUE CULTURE

[2] RecQ helicases: suppressors of tumorigenesis and premature aging [J].

Bachrati, CZ ;

Hickson, ID .

BIOCHEMICAL JOURNAL, 2003, 374 :577-606

[3] Arabidopsis RecQsim, a plant-specific member of the RecQ helicase family, can suppress the MMS hypersensitivity of the yeast sgs1 mutant [J].

Bagherieh-Najjar, MB ;

de Vries, OMH ;

Kroon, JTM ;

Wright, EL ;

Elborough, KM ;

Hille, J ;

Dijkwel, PP .

PLANT MOLECULAR BIOLOGY, 2003, 52 (02) :273-284

[4] Telomere instability in a human tumor cell line expressing a dominant-negative WRN protein [J].

Bai, YL ;

Murnane, JP .

HUMAN GENETICS, 2003, 113 (04) :337-347

[5] Switching and signaling at the telomere [J].

Blackburn, EH .

CELL, 2001, 106 (06) :661-673

[6] Severe developmental defects, hypersensitivity to DNA-damaging agents, and lengthened telomeres in Arabidopsis MRE11 mutants [J].

Bundock, P ;

Hooykaas, P .

PLANT CELL, 2002, 14 (10) :2451-2462

[7] Recombination-mediated lengthening of terminal telomeric repeats requires the Sgs1 DNA helicase [J].

Cohen, H ;

Sinclair, DA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (06) :3174-3179

[8] DNA damage-induced replication fork regression and processing in Escherichia coli [J].

Courcelle, J ;

Donaldson, JR ;

Chow, KH ;

Courcelle, CT .

SCIENCE, 2003, 299 (5609) :1064-1067

[9] Recombination at double-strand breaks and DNA ends: Conserved mechanisms from phage to humans [J].

Cromie, GA ;

Connelly, JC ;

Leach, DRF .

MOLECULAR CELL, 2001, 8 (06) :1163-1174

[10] Defining the roles of nucleotide excision repair and recombination in the repair of DNA interstrand cross-links in mammalian cells [J].

De Silva, IU ;

McHugh, PJ ;

Clingen, PH ;

Hartley, JA .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (21) :7980-7990

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