Cellular uptake of cationic lipid/DNA complexes by cultured myoblasts and myotubes

被引：14

作者：

Helbling-Leclerc, A ^{[1
]}

Scherman, D ^{[1
]}

Wils, P ^{[1
]}

机构：

[1] Rhone Poulenc Rorer, Ctr Rech Vitry Alfortville, CNRS, UMR133, F-94403 Vitry Sur Seine, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1418卷 / 01期

关键词：

gene transfer; cationic lipid; DNA; myoblast; myotube; cellular uptake;

D O I：

10.1016/S0005-2736(99)00012-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several cationic lipids which are highly efficient for delivering genes in vitro do not increase gene delivery in vivo after an intramuscular injection. In order to elucidate the origin of this phenomenon, we have studied the cellular uptake and intracellular fate of cationic lipid/DNA complexes in vitro on myogenic mouse cells (myoblasts and myotubes) of the C2 cell line and of primary cultures. We used a cationic lipid with a spermine head group and its fluorescent analog, and a fluorescent plasmid obtained by nick-translation. In myoblasts, transgene expression was obtained and lipoplexes were internalized in cytoplasmic vesicles. In myotubes, no transgene expression could be detected and we observed an absence of lipoplex internalization. The in vitro uptake of cationic lipid was inversely correlated with the degree of fusion of C2 cell myotubes cultures. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：165 / 175

页数：11

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