Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex

被引：930

作者：

Hamady, Micah ^{[2
]}

Walker, Jeffrey J. ^{[3
]}

Harris, J. Kirk ^{[4
,5
]}

Gold, Nicholas J. ^{[3
]}

Knight, Rob ^{[1
]}

机构：

[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

[2] Univ Colorado, Dept Comp Sci, Boulder, CO 80309 USA

[3] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA

[4] Univ Colorado, Dept Pediat, Aurora, CO 80045 USA

[5] Childrens Hosp, Ctr Hlth Sci, Aurora, CO 80045 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 03期

关键词：

D O I：

10.1038/NMETH.1184

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

引用

页码：235 / 237

页数：3

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