A rapid method to screen for cell-wall mutants using discriminant analysis of Fourier transform infrared spectra

被引:152
作者
Chen, LM
Carpita, NC
Reiter, WD
Wilson, RH
Jeffries, C
McCann, MC [1 ]
机构
[1] John Innes Ctr Plant Sci Res, Dept Cell Biol, Norwich NR4 7UH, Norfolk, England
[2] Univ E Anglia, Sch Phys, Norwich NR4 7TJ, Norfolk, England
[3] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA
[4] Univ Connecticut, Dept Mol & Cell Biol, Storrs, CT 06269 USA
[5] Univ Connecticut, Inst Sci Mat, Storrs, CT 06269 USA
[6] Inst Food Res, Dept Food Biophys, Norwich NR4 7UA, Norfolk, England
关键词
D O I
10.1046/j.1365-313x.1998.00301.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have developed a rapid method to screen large numbers of mutant plants for a broad range of cell wall phenotypes using Fourier transform infrared (FTIR) microspectroscopy of leaves. We established and validated a model that can discriminate between the leaves of wildtype and a previously defined set of cell-wall mutants of Arabidopsis. Exploratory principal component analysis indicated that mutants deficient in different cell-wall sugars can be distinguished from each other. Discrimination of cell-wall mutants from wild-type was independent of variability in starch content or additional unrelated mutations that might be present in a heavily mutagenised population. We then developed an analysis of FTIR spectra of leaves obtained from over 1000 mutagenised flax plants, and selected 59 plants whose spectral variation from wildtype was significantly out of the range of a wild-type population, determined by Mahalanobis distance. Cell wall sugars from the leaves of selected putative mutants were assayed by gas chromatography-mass spectrometry and 42 showed significant differences in neutral sugar composition. The FTIR spectra indicated that six of the remaining 17 plants have altered ester or protein content. We conclude that linear discriminant analysis of FTIR spectra is a robust method to identify a broad range of structural and architectural alterations in cell walls, appearing as a consequence of developmental regulation, environmental adaptation or genetic modification.
引用
收藏
页码:385 / 392
页数:8
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