Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages

被引：310

作者：

Abagnale, Giulio ^{[1
]}

Steger, Michael ^{[2
]}

Vu Hoa Nguyen ^{[3
]}

Hersch, Nils ^{[4
]}

Sechi, Antonio ^{[5
]}

Joussen, Sylvia ^{[1
]}

Denecke, Bernd ^{[6
]}

Merkel, Rudolf ^{[4
]}

Hoffmann, Bernd ^{[4
]}

Dreser, Alice ^{[7
]}

Schnakenberg, Uwe ^{[3
]}

Gillner, Arnold ^{[2
]}

Wagner, Wolfgang ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, Sch Med, Helmholtz Inst Biomed Engn Stem Cell Biol & Cellu, D-52074 Aachen, Germany

[2] Fraunhofer Inst Laser Technol, Aachen, Germany

[3] Rhein Westfal TH Aachen, Inst Mat Elect Engn IWE1 1, D-52074 Aachen, Germany

[4] Forschungszentrum Julich, Inst Complex Syst, Biomech ICS 7, D-52425 Julich, Germany

[5] Rhein Westfal TH Aachen, Sch Med, Inst Biomed Engn, Cell Biol, D-52074 Aachen, Germany

[6] Rhein Westfal TH Aachen, Sch Med, Interdisciplinary Ctr Clin Res, D-52074 Aachen, Germany

[7] Rhein Westfal TH Aachen, Sch Med, Inst Neuropathol, D-52074 Aachen, Germany

来源：

BIOMATERIALS | 2015年 / 61卷

关键词：

Mesenchymal stem cells; Microstructure; Nanotopography; Laser ablation; Gene expression; Osteogenesis; HUMAN PLATELET LYSATE; FETAL BOVINE SERUM; STROMAL CELLS; EXTRACELLULAR-MATRIX; FATE; NANOTOPOGRAPHY; GUIDANCE; BIOMATERIALS; LITHOGRAPHY; EXPRESSION;

D O I：

10.1016/j.biomaterials.2015.05.030

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Surface topography impacts on cell growth and differentiation, but it is not trivial to generate defined surface structures and to assess the relevance of specific topographic parameters. In this study, we have systematically compared in vitro differentiation of mesenchymal stem cells (MSCs) on a variety of groove/ridge structures. Micro- and nano-patterns were generated in polyimide using reactive ion etching or multi beam laser interference, respectively. These structures affected cell spreading and orientation of human MSCs, which was also reflected in focal adhesions morphology and size. Time-lapse demonstrated directed migration parallel to the nano-patterns. Overall, surface patterns clearly enhanced differentiation of MSCs towards specific lineages: 15 gm ridges increased adipogenic differentiation whereas 2 gm ridges enhanced osteogenic differentiation. Notably, nano-patterns with a periodicity of 650 nm increased differentiation towards both osteogenic and adipogenic lineages. However, in absence of differentiation media surface structures did neither induce differentiation, nor lineage-specific gene expression changes. Furthermore, nanostructures did not affect the YAP/TAZ complex, which is activated by substrate stiffness. Our results provide further insight into how structuring of tailored biomaterials and implant interfaces - e.g. by multi beam laser interference in sub-micrometer scale do not induce differentiation of MSCs per se, but support their directed differentiation. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：316 / 326

页数：11

共 66 条

[1]

Spatial control of adult stem cell fate using nanotopographic cues [J].