Effects of bisphosphonates on alkaline phosphatase activity, mineralization, and prostaglandin E(2) synthesis in the clonal osteoblast-like cell line MC3T3-E1

被引：50

作者：

Igarashi, K ^{[1
]}

Hirafuji, M ^{[1
]}

Adachi, H ^{[1
]}

Shinoda, H ^{[1
]}

Mitani, H ^{[1
]}

机构：

[1] TOHOKU UNIV, SCH DENT, DEPT PHARMACOL, AOBA KU, SENDAI, MIYAGI 98077, JAPAN

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1997年 / 56卷 / 02期

关键词：

D O I：

10.1016/S0952-3278(97)90508-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of 3 bisphosphonates, AHBuBP, AHPrBP, and Cl(2)MBP on cell growth, alkaline phosphatase (ALP) activity, mineralization, and prostaglandin E(2) (PGE(2)) synthesis in the clonal osteoblast-like cell line MC3T3-E1 were studied. These bisphosphonates had essentially similar effects on growth and the osteoblastic functions of the cells, i.e., they had no inhibitory effects on cell growth except at higher concentrations, they increased ALP activity, and inhibited PGE(2) production. In the presence of AHBuBP, ALP activity was higher than that in the control after day 6 of culture. Lower concentrations of AHBuBP slightly facilitated mineralization by the cells. It is probable that bisphosphonates enhance the functions of osteoblasts in certain concentration and that the inhibition of endogenous PGE(2) production may be involved in the mechanism of action of bisphosphonates.

引用

页码：121 / 125

页数：5

共 24 条

[1] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].

BURTON, K .

BIOCHEMICAL JOURNAL, 1956, 62 (02) :315-323

[2] BISPHOSPHONATES DIRECTLY INHIBIT THE BONE-RESORPTION ACTIVITY OF ISOLATED AVIAN OSTEOCLASTS INVITRO [J].

CARANO, A ;

TEITELBAUM, SL ;

KONSEK, JD ;

SCHLESINGER, PH ;

BLAIR, HC .

JOURNAL OF CLINICAL INVESTIGATION, 1990, 85 (02) :456-461

[3]

ENDE JJ, 1978, P K NED AKAD C BIOL, V81, P150

[4]

FELIX R, 1979, BIOCHEM J, V183, P73, DOI 10.1042/bj1830073

[5] DICHLOROMETHYLENEBISPHOSPHONATE (CL2MBP) INHIBITS BONE-RESORPTION THROUGH INJURY TO OSTEOCLASTS THAT RESORB CL2MBP-COATED BONE [J].

FLANAGAN, AM ;

CHAMBERS, TJ .

BONE AND MINERAL, 1989, 6 (01) :33-43

[6] BISPHOSPHONATES - PHARMACOLOGY AND USE IN THE TREATMENT OF TUMOR-INDUCED HYPERCALCEMIC AND METASTATIC BONE-DISEASE [J].

FLEISCH, H .

DRUGS, 1991, 42 (06) :919-944

[7]

Fleisch H., 1993, BISPHOSPHONATES BONE

[8] ROLE OF ENDOGENOUS PGE(2) IN OSTEOBLASTIC FUNCTIONS OF A CLONAL OSTEOBLAST-LIKE CELL, MC3T3-E1 [J].

IGARASHI, K ;

HIRAFUJI, M ;

ADACHI, H ;

SHINODA, H ;

MITANI, H .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1994, 50 (04) :169-172

[9]

LOWRY OH, 1954, J BIOL CHEM, V207, P19

[10]

MORITA I, 1989, ADV PROSTAG THROMB L, V19, P419

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