Steroid hormone receptor-mediated histone deacetylation and transcription at the mouse mammary tumor virus promoter

被引：35

作者：

Sheldon, LA

Becker, M

Smith, CL

机构：

[1] Dartmouth Coll Sch Med, Dept Physiol, Lebanon, NH 03756 USA

[2] NCI, Lab Receptor Biol & Gene Express, Canc Res Ctr, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 35期

关键词：

D O I：

10.1074/jbc.C100315200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetylation of lysines in histones H3 and H4 N-terminal tails is associated with transcriptional activation and deacetylation with repression. Our studies with the mouse mammary tumor virus (MMTV) promoter in chromatin show significant levels of acetylation at promoter proximal and distal regions prior to transactivation. Upon activation with glucocorticoids or progestins, promoter proximal histones become deacetylated within the region of inducible nuclease hypersensitivity. The deacetylation lags behind the initiation of transcription, indicating a role in post-activation regulation. Our results indicate a novel mechanism by which target promoters are regulated by steroid receptors and chromatin modification machinery.

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收藏

页码：32423 / 32426

页数：4

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