Interleukin-3 production by mast cells from human lung

被引:15
作者
Ishizuka, T [1 ]
Okayama, Y [1 ]
Kobayashi, H [1 ]
Mori, M [1 ]
机构
[1] Gunma Univ, Sch Med, Dept Internal Med 1, Maebashi, Gumma 3718511, Japan
关键词
D O I
10.1023/A:1020235400073
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The cytokine interleukin (IL)-3 is important in the proliferation of eosinophils and basophils in the airway. We investigated IL-3 production by human lung mast cells as a possible mechanism of the airway inflammation constituting the late asthmatic response. Mast cells were purified using affinity magnetic selection with the monoclonal antibody YB5.B8 and then stimulated with anti-human IgE antibody. IL-3 release was detectable by enzyme-linked immunosorbent assay 8 h after anti-IgE stimulation. IL-3 release 24 h after anti-IgE stimulation was significantly greater than its controls. By reverse transcription-polymerase chain reaction, IL-3 mRNA was detected weakly 2 h after anti-IgE stimulation, peaking at 4 h and waning at 8 h. Immunocytochemistry to localize IL-3 demonstrated mast cell staining. These results suggest that mast cells release IL-3 in response to high-affinity IgE receptor.
引用
收藏
页码:25 / 35
页数:11
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