A concise synthesis of the Pennsylvania green fluorophore and labeling of intracellular targets with O6-benzylguanine derivatives

被引：32

作者：

Mottram, Laurie F.

Maddox, Ewa

Schwab, Markus

Beaufils, Florent

Peterson, Blake R.

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

[2] Covalys Biosci AG, CH-4108 Witterswil, Switzerland

来源：

ORGANIC LETTERS | 2007年 / 9卷 / 19期

关键词：

D O I：

10.1021/ol7015093

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We report improved syntheses of the Pennsylvania Green and 4-carboxy-Pennsylvania Green fluorophores; the latter compound was prepared from methyl 4-iodo-3-methylbenzoate in a three-pot process (32% overall yield). Chinese hamster ovary cells expressing O-6-alkylguanine-DNA alkyltransferase fusion proteins were treated with Pennsylvania Green and Oregon Green linked to O-6-benzyiguanine (SNAP-Tag substrates). Analysis of living cells by confocal microscopy revealed that Pennsylvania Green derivatives exhibit substantially higher cell permeability than analogous Oregon Green-derived molecular probes.

引用

收藏

页码：3741 / 3744

页数：4

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