Regulation of platelet granule exocytosis by S-nitrosylation

被引：112

作者：

Morrell, CN

Matsushita, K

Chiles, K

Scharpf, RB

Yamakuchi, M

Mason, RJA

Bergmeier, W

Mankowski, JL

Baldwin, WM

Faraday, N

Lowenstein, CJ

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Comparat Med, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Dept Anesthesiol, Baltimore, MD 21205 USA

[5] Harvard Univ, Sch Med, Ctr Blood Res, Boston, MA 02115 USA

[6] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 10期

关键词：

alpha-granules; nitric oxide;

D O I：

10.1073/pnas.0408310102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nitric oxide (NO) regulates platelet activation by cGMP-dependent mechanisms and by mechanisms that are not completely defined. Platelet activation includes exocytosis of platelet granules, releasing mediators that regulate interactions between platelets, leukocytes, and endothelial cells. Exocytosis is mediated in part by N-ethylmaleimide-sensitive factor (NSF), an ATPase that disassembles complexes of soluble NSF attachment protein receptors. We now demonstrate that NO inhibits exocytosis of dense granules, lysosomal granules, and a-granules from human platelets by S-nitrosylation of NSF. Platelets lacking endothelial NO synthase show increased rolling on venules, increased thrombosis in arterioles, and increased exocytosis in vivo. Regulation of exocytosis is thus a mechanism by which NO regulates thrombosis.

引用

页码：3782 / 3787

页数：6

共 56 条

[1] Inhaled NO inhibits platelet aggregation and elevates plasma but not intraplatelet cGMP in healthy human volunteers
Beghetti, M
Sparling, C
Cox, PN
Stephens, D
Adatia, I
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2003, 285 (02): : H637 - H642
[2] PURIFICATION OF AN N-ETHYLMALEIMIDE-SENSITIVE PROTEIN CATALYZING VESICULAR TRANSPORT
BLOCK, MR
GLICK, BS
WILCOX, CA
WIELAND, FT
ROTHMAN, JE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) : 7852 - 7856
[3] INHIBITION OF HUMAN PLATELET REACTIVITY BY ENDOTHELIUM-DERIVED RELAXING FACTOR FROM HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS IN SUSPENSION - BLOCKADE OF AGGREGATION AND SECRETION BY AN ASPIRIN-INSENSITIVE MECHANISM
BROEKMAN, MJ
EIROA, AM
MARCUS, AJ
[J]. BLOOD, 1991, 78 (04) : 1033 - 1040
[4] PROPERTIES OF A NOVEL NITRIC OXIDE-STIMULATED ADP-RIBOSYLTRANSFERASE
BRUNE, B
LAPETINA, EG
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 279 (02) : 286 - 290
[5] BUSSE R, 1987, N-S ARCH PHARMACOL, V336, P566
[6] Antithrombotic activity of TNF-α
Cambien, B
Bergmeier, W
Saffaripour, S
Mitchell, HA
Wagner, DD
[J]. JOURNAL OF CLINICAL INVESTIGATION, 2003, 112 (10) : 1589 - 1596
[7] Endothelial nitric oxide synthase: the Cinderella of inflammation?
Cirino, G
Fiorucci, S
Sessa, WC
[J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 2003, 24 (02) : 91 - 95
[8] Cyclic GMP-dependent protein kinases and the cardiovascular system
Feil, R
Lohmann, SM
de Jonge, H
Walter, U
Hofmann, F
[J]. CIRCULATION RESEARCH, 2003, 93 (10) : 907 - 916
[9] Proteins of the exocytotic core complex mediate platelet α-granule secretion -: Roles of vesicle-associated membrane protein, SNAP-23, and syntaxin 4
Flaumenhaft, R
Croce, K
Chen, E
Furie, B
Furie, BC
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (04) : 2492 - 2501
[10] Freedman JE, 1999, CIRC RES, V84, P1416

← 1 2 3 4 5 6 →