Escherichia coli strain for thermoinducible T7 RNA polymerase-driven expression

被引：3

作者：

Fedorova, ND

Peredelchuk, MY

Kirpichnikov, MP

Bennett, GN

机构：

[1] RICE UNIV,DEPT BIOCHEM & CELL BIOL,HOUSTON,TX 77005

[2] VA ENGELHARDT MOL BIOL INST,MOSCOW 117894,RUSSIA

来源：

GENE | 1996年 / 177卷 / 1-2期

关键词：

recombinant DNA; phage lambda; site-specific recombination; integrase; attachment site;

D O I：

10.1016/0378-1119(96)00294-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An Escherichia coli strain for thermoinducible T7Pol-driven transcription has been constructed. The strain was obtained by site-specific integration of the T7 gene 1 coding for T7Pol into the attB site of phage lambda in the E. coli chromosome. The expression of the inserted gene is regulated by clts857 and major early promoter-operator regions of phage lambda.

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收藏

页码：267 / 268

页数：2

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