Color control of natural fluorescent proteins by photonic crystals

被引:45
作者
Blum, Christian [1 ,2 ]
Mosk, Allard P. [2 ,3 ]
Nikolaev, Ivan S. [4 ]
Subramaniam, Vinod [1 ,2 ]
Vos, Willem L. [2 ,3 ,4 ]
机构
[1] Univ Twente, Fac Sci & Technol, NL-7500 AE Enschede, Netherlands
[2] MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands
[3] Univ Twente, Fac Sci & Technol, Complex Photon Syst COPS, NL-7500 AE Enschede, Netherlands
[4] Ctr Nanophoton, FOM, Inst Atom & Mol Phys AMOLF, NL-1098 SJ Amsterdam, Netherlands
关键词
biophotonic engineering; emission control; fluorescent proteins; nanomaterials; photonic crystals;
D O I
10.1002/smll.200701160
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The emission of fluorescent proteins inside photonic crystals is studied. It is demonstrated that the apparent emission color of the fluorescent protein can be controlled externally by the photonic crystal. With increasing crystal lattice parameter, the appearance of the proteins turns from orange to red, and suddenly to green. The dramatic color changes agree with the theoretically expected redistribution of light escaping around the stop band of the photonic crystal. Our experiments show the potential of combining biological systems with nanophotonics. This "biophotonic engineering" may be extended to control emission rates and complex Forster energy-transfer systems obtained by protein engineering.
引用
收藏
页码:492 / 496
页数:5
相关论文
共 27 条
[1]   Deterministic coupling of single quantum dots to single nanocavity modes [J].
Badolato, A ;
Hennessy, K ;
Atatüre, M ;
Dreiser, J ;
Hu, E ;
Petroff, PM ;
Imamoglu, A .
SCIENCE, 2005, 308 (5725) :1158-1161
[2]   Single oligomer spectra probe chromophore nanoenvironments of tetrameric fluorescent proteins [J].
Blum, Christian ;
Meixner, Alfred J. ;
Subramaniam, Vinod .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (26) :8664-8670
[3]   GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].
CHALFIE, M ;
TU, Y ;
EUSKIRCHEN, G ;
WARD, WW ;
PRASHER, DC .
SCIENCE, 1994, 263 (5148) :802-805
[4]   Microwave photodielectric and photoconductivity studies of commercial titanium dioxide pigments: the influence of transition metal dopants [J].
Janes, R ;
Edge, M ;
Robinson, J ;
Allen, NS ;
Thompson, F .
JOURNAL OF MATERIALS SCIENCE, 1998, 33 (12) :3031-3036
[5]   Light exiting from real photonic band gap crystals is diffuse and strongly directional [J].
Koenderink, AF ;
Vos, WL .
PHYSICAL REVIEW LETTERS, 2003, 91 (21)
[6]   Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Forster radius [J].
Kremers, Gert-Jan ;
Goedhart, Joachim ;
van Munster, Erik B. ;
Gadella, Theodorus W. J., Jr. .
BIOCHEMISTRY, 2006, 45 (21) :6570-6580
[7]   Development and use of fluorescent protein markers in living cells [J].
Lippincott-Schwartz, J ;
Patterson, GH .
SCIENCE, 2003, 300 (5616) :87-91
[8]   Controlling the dynamics of spontaneous emission from quantum dots by photonic crystals [J].
Lodahl, P ;
van Driel, AF ;
Nikolaev, IS ;
Irman, A ;
Overgaag, K ;
Vanmaekelbergh, D ;
Vos, WL .
NATURE, 2004, 430 (7000) :654-657
[9]   Fluorescent proteins from nonbioluminescent Anthozoa species [J].
Matz, MV ;
Fradkov, AF ;
Labas, YA ;
Savitsky, AP ;
Zaraisky, AG ;
Markelov, ML ;
Lukyanov, SA .
NATURE BIOTECHNOLOGY, 1999, 17 (10) :969-973
[10]   Bragg diffraction from indium phosphide infilled fcc silica colloidal crystals [J].
Míguez, H ;
Blanco, A ;
Meseguer, F ;
López, C ;
Yates, HM ;
Pemble, ME ;
Fornés, V ;
Mifsud, A .
PHYSICAL REVIEW B, 1999, 59 (03) :1563-1566