Carbon dioxide storage and nonbicarbonate buffering in the human body before and after an Himalayan expedition

被引:3
作者
Böning, D
Maassen, N
Steinacker, JM
Seifert, C
Thomas, A
Schmidt, W
机构
[1] Free Univ Berlin, Dept Sports Med, Univ Hosp Benjamin Franklin, D-14195 Berlin, Germany
[2] Hannover Med Sch, Dept Sports & Exercise Physiol, D-30625 Hannover, Germany
[3] Univ Ulm, Dept Sports Med, D-89070 Ulm, Germany
[4] Armed Forces Hosp Ulm, D-89070 Ulm, Germany
关键词
osmolality; erythrocyte age; altitude; hypercapnia; hypocapnia;
D O I
10.1007/s004210050537
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Before and 7-12 days after an Himalayan expedition CO2 equilibration curves were determined in the blood plasma of 12 mountaineers by in vitro and in vivo CO2 titration; in vivo osmolality changes (Delta Osm . Delta PCO2-1, Delta Osm . Delta pH(-1), where PCO2 is the partial pressure of CO2) during the latter experiments yielded estimates of whole body CO2 storage. In vitro -Delta[HCO3-] . Delta pH(-1) [nonbicarbonate buffer capacity (beta) of blood] was increased 7 days after descent [before 31.3 (SEM 0.4) mmol . kgH(2)O(-1), after 38.3 (SEM 3.9) mmol . kgH(2)O(-1); P < 0.05] resulting from an increased proportion of young: erythrocytes; in additional experiments an augmented P was found in young (low density cells) compared to old cells [(1.097 g . ml(-1): 0.216 (SEM 0.098) mmol . gHb(-1), > 1.100 g . ml(-1): 0.145 (SEM 0.013) mmol . gHb(-1), where Hb is haemoglobin; P<0.02]. In spite of increased Hb mass in vivo Delta[CO2total] . Delta PCO2-1 [0.192 (SEM 0.010)mmol . kgH(2)O(-1) . mmHg(-1)] and -Delta[HCO3-] . Delta pH(-1) [17.9 (SEM 1.0) mmol . kgH(2)O(-1)] as indicators of extracellular beta rose only slightly after altitude (7 days + 16%, P<0.02; +7%, NS) because of haemodilution. The Delta Osm . Delta P-CO2(-1) [0.230(SEM 0.015) mosmol . kgH(2)O(-1) . mmHg(-1)] remained unchanged. Prealtitude differences in Delta Osm . Delta pH(-1) between hypercapnia [-41(SEM 5)mosmol . kgH(2)O(-1)] and hypocapnia [-20 (SEM 3) mosmol kgH(2)O(-1) P<0.01] disappeared temporarily after return since the former slope was reduced. The high value during hypercapnia before ascent probably resulted from mechanisms stabilizing intracellular pH during moderate hypercapnia which were attenuated after descent.
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页码:457 / 466
页数:10
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