Nanospheres for DNA separation chips

被引：106

作者：

Tabuchi, M

Ueda, M

Kaji, N

Yamasaki, Y

Nagasaki, Y

Yoshikawa, K

Kataoka, K

Baba, Y

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Dept Med Chem, Tokushima 7708505, Japan

[2] Univ Tokyo, Grad Sch Engn, Dept Mat Sci, Bunkyo Ku, Tokyo 1138656, Japan

[3] Tokyo Univ Sci, Dept Mat Sci & Technol, Noda, Chiba 2788510, Japan

[4] Kyoto Univ, Grad Sch Sci, Dept Phys, Kyoto 6068502, Japan

[5] JST, Japan Sci & Technol Agcy, CREST, Tokyo, Japan

[6] Univ Tokushima, 21st Century COE Program, Tokushima 770, Japan

[7] AIST, Natl Inst Adv Ind Sci & Technol, Single Mol Bioanal Lab, Takamatsu, Kagawa 7610395, Japan

来源：

NATURE BIOTECHNOLOGY | 2004年 / 22卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1038/nbt939

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report here a technology to carry out separations of a wide range of DNA fragments with high speed and high resolution. The approach uses a nanoparticle medium, core-shell type nanospheres, in conjunction with a pressurization technique during microchip electrophoresis. DNA fragments up to 15 kilobase pairs (kbp) were successfully analyzed within 100 s without observing any saturation in migration rates. DNA fragments migrate in the medium while maintaining their characteristic molecular structure. To guarantee effective DNA loading and electrofocusing in the nanosphere solution, we developed a double pressurization technique. Optimal pressure conditions and concentrations of packed nanospheres are critical to achieve improved DNA separations.

引用

页码：337 / 340

页数：4

共 22 条

[1] A TRANSIENT ENTANGLEMENT COUPLING MECHANISM FOR DNA SEPARATION BY CAPILLARY ELECTROPHORESIS IN ULTRADILUTE POLYMER-SOLUTIONS [J].