Chromatin immunoprecipitation: a tool for studying histone acetylation and transcription factor binding

被引：140

作者：

Spencer, VA ^{[1
]}

Sun, JM ^{[1
]}

Li, L ^{[1
]}

Davie, JR ^{[1
]}

机构：

[1] Univ Manitoba, Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada

来源：

METHODS | 2003年 / 31卷 / 01期

基金：

加拿大健康研究院;

关键词：

chromatin immunoprecipitation; histone; transcription factor; formaldehyde; cisplatin;

D O I：

10.1016/S1046-2023(03)00089-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The function of a protein in gene expression can often be explained, in part, by the location of that protein along a specific gene sequence. In recent years, the chromatin immunoprecipitation (Chip) assay has been developed to study the association of proteins located within 2 A of DNA such as transcription factors and modified histones. Numerous important findings have been published using the ChIP assay and many questions about transcription have been answered. In this article, we present the Chip assay currently used in our lab and discuss the various ways to optimize this assay for one's own use. (C) 2003 Published by Elsevier Science (USA).

引用

页码：67 / 75

页数：9

共 21 条

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