Cellulose-binding domain of endoglucanase III from Trichoderma reesei disrupting the structure of cellulose

被引：65

作者：

Xiao, ZZ ^{[1
]}

Gao, PJ ^{[1
]}

Qu, YB ^{[1
]}

Wang, TH ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 09期

基金：

中国国家自然科学基金;

关键词：

cellulose-binding domain; endoglucanase III; Trichoderma reesei;

D O I：

10.1023/A:1010325122851

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The DNA fragment encoding the cellulose binding domain of endoglucanase III (CBDEG III) from Trichoderma reesei was subcloned and expressed in E. coli. The CBDEG III had a high affinity for cellulose. The morphological and structural changes of cellulose after treatment with CBDEG III indicated a 17% decrease in number of hydrogen bonds and a 16.5% decrease in crystalline index. X-ray diffraction and IR spectra analyses indicated that the destabilization and breakage of the hydrogen bonds in crystalline cellulose accounted for the non-hydrolytic disruption of the structure of cellulose.

引用

页码：711 / 715

页数：5

共 12 条

[1]

Gao PJ, 1998, PROG NAT SCI, V8, P117

[2]

Gao PJ, 2001, ACTA BIOCH BIOPH SIN, V33, P13

[3] Adsorption behaviors of two cellobiohydrolases and their core proteins from Trichoderma reesei on Avicel PH 101 [J].

Kim, DW ;

Jang, YH ;

Jeong, YK .

BIOTECHNOLOGY LETTERS, 1997, 19 (09) :893-897

[4] The cellulases endoglucanase I and cellobiohydrolase II of Trichoderma reesei act synergistically to solubilize native cotton cellulose but not to decrease its molecular size [J].

KlemanLeyer, KM ;

SiikaAho, M ;

Teeri, TT ;

Kirk, TK .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (08) :2883-2887

[5] The cellulose-binding domain of the major cellobiohydrolase of Trichoderma reesei exhibits true reversibility and a high exchange rate on crystalline cellulose [J].

Linder, M ;

Teeri, TT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (22) :12251-12255

[6] The roles and function of cellulose-binding domains [J].

Linder, M ;

Teeri, TT .

JOURNAL OF BIOTECHNOLOGY, 1997, 57 (1-3) :15-28

[7] Characterization of a double cellulose-binding domain - Synergistic high affinity binding to crystalline cellulose [J].

Linder, M ;

Salovuori, I ;

Ruohonen, L ;

Teeri, TT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (35) :21268-21272

[8] Solution structure of the cellulose-binding domain of endoglucanase I from Trichoderma reesei and its interaction with cello-oligosaccharides [J].

Mattinen, ML ;

Linder, M ;

Drakenberg, T ;

Annila, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 256 (02) :279-286

[9] EGIII, A NEW ENDOGLUCANASE FROM TRICHODERMA-REESEI - THE CHARACTERIZATION OF BOTH GENE AND ENZYME [J].

SALOHEIMO, M ;

LEHTOVAARA, P ;

PENTTILA, M ;

TEERI, TT ;

STAHLBERG, J ;

JOHANSSON, G ;

PETTERSSON, G ;

CLAEYSSENS, M ;

TOMME, P ;

KNOWLES, JKC .

GENE, 1988, 63 (01) :11-21

[10] Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose [J].

Srisodsuk, M ;

Lehtio, J ;

Linder, M ;

MargollesClark, E ;

Reinikainen, T ;

Teeri, TT .

JOURNAL OF BIOTECHNOLOGY, 1997, 57 (1-3) :49-57

← 1 2 →