Global gene expression analysis of Anopheles gambiae responses to microbial challenge

被引：91

作者：

Aguilar, R ^{[1
]}

Jedlicka, AE ^{[1
]}

Mintz, M ^{[1
]}

Mahairaki, V ^{[1
]}

Scott, AL ^{[1
]}

Dimopoulos, G ^{[1
]}

机构：

[1] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, W Harry Feinstone Dept Mol Microbiol & Immunol, Baltimore, MD 21205 USA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2005年 / 35卷 / 07期

关键词：

anopheles; transcriptome; immune response;

D O I：

10.1016/j.ibmb.2005.02.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anopheles gambiae transcript responses to experimental challenge with heat inactivated Salmonella typhimurium, Staphylococcus aureus and Beauveria bassiana have been analyzed with an Affymetrix GeneChip comprising the entire predicted mosquito transcriptome. Significant up- or down-regulation (greater than 2-fold) can be assayed for approximately 2% of the mosquito transcriptome and affected genes represent a variety of functional classes that include immunity, apoptosis, stress response, detoxification, metabolism, blood digestion, olfaction and others. Transcript responses to the 3 microbial elicitors exhibit an exceptionally high degree of specificity and only a few genes are significantly regulated by more than 1 of the tested elicitors. This study identifies several transcripts that have not been linked directly to immune response in A. gambiae previously; their infection responsiveness and sequence feature; do however suggest implication in defence reactions; examples are genes encoding leucine-rich repeat domain proteins, cuticle domain proteins and proteins containing immunoglobulin and fibronectin domains. (c) 2005 Elsevier Ltd. All rights reserved.

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收藏

页码：709 / 719

页数：11

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