The fungicide phosphonate disrupts the phosphate-starvation response in Brassica nigra seedlings

被引:100
作者
Carswell, C
Grant, BR
Theodorou, ME
Harris, L
Niere, JO
Plaxton, WC
机构
[1] QUEENS UNIV, DEPT BIOL, KINGSTON, ON K7L 3N6, CANADA
[2] QUEENS UNIV, DEPT BIOCHEM, KINGSTON, ON K7L 3N6, CANADA
[3] UNIV MELBOURNE, DEPT BIOCHEM, PARKVILLE, VIC 3052, AUSTRALIA
[4] ROYAL MELBOURNE INST TECHNOL, DEPT APPL CHEM, BUNDOORA, VIC 3083, AUSTRALIA
关键词
D O I
10.1104/pp.110.1.105
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The development of Brassica nigra seedlings over 20 d of growth was disrupted by the fungicide phosphonate (Phi) in a manner inversely correlated with nutritional inorganic phosphate (Pi) levels. The growth of Pi-sufficient (1.25 mM Pi) seedlings was suppressed when 10, but not 5, mM Phi was added to the nutrient medium. In contrast, the fresh weights and root:shoot ratios of Pi-limited (0.15 mM) seedlings were significantly reduced;at 1.5 mM Phi, and they progressively declined to about 40% of control values as medium Phi concentration was increased to 10 mM. Intracellular Pi levels generally decreased in Phi-treated seedlings, and Phi accumulated in leaves and roots to levels up to 6- and 16-fold that of Pi in Pi-sufficient and Pi-limited plants, respectively. Extractable activities of the Pi-starvation-inducible enzymes phosphoenolpyruvate phosphatase and inorganic pyrophosphate-dependent phosphofructokinase were unaltered in Pi-sufficient seedlings grown on 5 or 10 mM Phi. However, when Pi-limited seedlings were grown on 1.5 to 10 mM PHi (a) the induction of phosphoenolpyruvate phosphatase and inorganic pyrophosphate-dependent phosphofructokinase activities by Pi limitation was reduced by 40 to 90%, whereas (b) soluble protein concentrations and the activities of the ATP-dependent phosphofructokinase and pyruvate kinase were unaffected. It is concluded that Phi specifically interrupts processes involved in regulation of the Pi-starvation response in B. nigra.
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页码:105 / 110
页数:6
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