Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR

被引：14

作者：

Desai, Prerak T. ^{[1
]}

Walsh, Marie K. ^{[1
]}

Weimer, Bart C. ^{[1
]}

机构：

[1] Utah State Univ, Dept Nutr & Food Sci, Ctr Integrated BioSyst, Western Dairy Ctr,Ctr Microbe Detect & Physiol, Logan, UT 84322 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 07期

关键词：

D O I：

10.1128/AEM.02601-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of similar to 4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.

引用

页码：2254 / 2258

页数：5

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