Characterisation of the Roundup Ready soybean insert

被引：109

作者：

Windels, P

Taverniers, I

Depicker, A

Van Bockstaele, E

De Loose, M

机构：

[1] Agr Res Ctr, Dept Genet & Plant Breeding, B-9090 Melle, Belgium

[2] State Univ Ghent, Dept Mol Genet, B-9000 Ghent, Belgium

[3] State Univ Ghent, Dept Plant Prod, B-9000 Ghent, Belgium

[4] Hogesch Gent, Comm Res & Serv & Arts, B-9000 Ghent, Belgium

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2001年 / 213卷 / 02期

关键词：

Roundup Ready soybean; junction fragment; rearrangements; GMO; identification;

D O I：

10.1007/s002170100336

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this article we describe the isolation and characterisation of the junction between insert DNA and plant DNA in the transgenic Roundup Ready soybean line event 40-3-2. Our results establish that during integration of the insert DNA several rearrangements occurred at the 3' NOS junction and that the genomic plant DNA at the pre-integration site may have been rearranged. These findings highlight the utility of characterising junction regions to fulfil the request for information regarding which DNA sequences have been incorporated in commercialised transgenic lines. Furthermore, the characterisation of junction regions is, in our opinion, the method of choice to support method development for detection and identification of plant biotechnology-derived products.

引用

页码：107 / 112

页数：6

共 19 条

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[3]

Depicker A., 1998, Mededelingen Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen Universiteit Gent, V63, P1479

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