Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin

被引：48

作者：

Francia, MV

Haas, W

Wirth, R

Samberger, E

Muscholl-Silberhorn, A

Gilmore, MS

Ike, Y

Weaver, KE

An, FY

Clewell, DB ^{[1
]}

机构：

[1] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA

[3] Univ Oklahoma, Hlth Sci Ctr, Dept Ophthalmol, Oklahoma City, OK 73104 USA

[4] Univ Oklahoma, Hlth Sci Ctr, Dept Microbiol, Oklahoma City, OK 73104 USA

[5] Univ Regensburg, Dept Microbiol, D-9053 Regensburg, Germany

[6] Gunma Univ, Sch Med, Dept Microbiol, Maebashi, Gumma 371, Japan

[7] Univ S Dakota, Dept Microbiol, Vermillion, SD 57069 USA

来源：

PLASMID | 2001年 / 46卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1006/plas.2001.1533

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

pAD1 is a 59.3-kb plasmid in Enterococcus faecalis that has been the subject of intense investigation with regard to its pheromone-inducible conjugation behavior as well as its contribution to virulence. Approximately two-thirds of the pAD1 nucleotide sequence has been previously reported. Here we report on an analysis of the final similar to 22 kb, a significant portion of which is believed to encode structural genes associated with conjugation. The conjugation-regulated region was also found to contain a new (second) origin of conjugative transfer (oriT). A list of open reading frames covering the entire plasmid is presented. (C) 2001 Academic Press.

引用

页码：117 / 127

页数：11

共 59 条

[1] CHARACTERIZATION OF THE DETERMINANT (TRAB) ENCODING SEX-PHEROMONE SHUTDOWN BY THE HEMOLYSIN BACTERIOCIN PLASMID-PAD1 IN ENTEROCOCCUS-FAECALIS [J].