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Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells

被引：21
作者：
Kovacs, JR
Zheng, Y
Shen, HM
Meng, WS
机构：
[1] Duquesne Univ, Div Pharmaceut Sci, Pittsburgh, PA 15282 USA
[2] Univ Pittsburgh, Dept Radiat Oncol, Pittsburgh, PA 15260 USA
[3] Univ Pittsburgh, Inst Canc, Pittsburgh, PA 15260 USA
来源：
BIOMATERIALS | 2005年 / 26卷 / 33期
关键词：
DNA delivers; dendritic cell; nanoparticle; cationic peptide; oligonucleotide;
D O I：
10.1016/j.biomaterials.2005.04.022
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 [生物医学工程];
摘要：
The aim of this study is to evaluate a novel microspheric vector for delivery of oligonucleotides (ODN) into dendritic cells (DC). A requirement of decoy-based modulation of transcriptional activities ill DC is that the ODN would ha c to accumulate inside the cell, Using an ex vivo DC culture model. we demonstrate that anionic microspheres (MS) coated with an ornithine histadine-based cationic peptide (O10H6) is an effective carrier of short ODN. This method does not disrupt the colloidal nature of the microspheric particles. The MS provide stabilizing effect on DNA and O10H6 complexation. Accumulation of ODN in DC is greatly enhanced with the Surface modified MS. Taken together, these data demonstrate that the self assembly System of MSO10H6 is an effective delivery vehicle for DNA-based modulation of DC functions. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：6754 / 6761
页数：8
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