Adsorptive voltammetric behavior of thymine in presence of guanine at the static mercury drop electrode

被引：17

作者：

Farias, PAM ^{[1
]}

Wagener, ADR ^{[1
]}

Castro, AA ^{[1
]}

机构：

[1] Pontificia Univ Catolica Rio de Janeiro, Dept Chem, BR-22453900 Rio De Janeiro, Brazil

来源：

ANALYTICAL LETTERS | 2001年 / 34卷 / 08期

关键词：

thymine determination; guanine; acridine orange; copper; ssDNA; stripping voltammetry;

D O I：

10.1081/AL-100104154

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical stripping procedure for trace measurements of thymine is described by adsorption on a static mercury drop electrode. Cyclic voltammetry was used to characterize the interfacial and redox behavior. Optimum experimental conditions were found to be the use of a 0.005 M NaOH solution, an initial potential of -0.08 V, a scan rate of 100 mV.s(-1) and a linear scan mode. The response of free thymine is linear over the concentration range 0.1-0.5 ppm. Linear scan voltammetry, without acumulation time yields a detection limit of 62 ppb thymine. Acridine orange and copper were also investigated for measuring thymine in the presence of guanine.

引用

页码：1295 / 1310

页数：16

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