The cytotoxic activity of Bacillus anthracis lethal factor is inhibited by leukotriene A(4) hydrolase and metallopeptidase inhibitors

被引：35

作者：

Menard, A

Papini, E

Mock, M

Montecucco, C

机构：

[1] UNIV PADUA, CNR, CTR BIOMEMBRANE, I-35121 PADUA, ITALY

[2] UNIV PADUA, DIPARTIMENTO SCI BIOMED, I-35121 PADUA, ITALY

[3] INST PASTEUR, GENET MOL TOXINES LAB, CNRS, URA 1858, F-75724 PARIS 15, FRANCE

来源：

BIOCHEMICAL JOURNAL | 1996年 / 320卷

关键词：

D O I：

10.1042/bj3200687

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lethal factor of Bacillus anthracis is central to the pathogenesis of anthrax. Its mechanism of action is still unknown. Recently, on the basis of sequence similarities, we suggested that lethal factor might act similarly to leukotriene A(4) hydrolase (LTA(4)), a bifunctional enzyme also endowed with a metallopeptidase activity. Here we show that some inhibitors of the LTA(4) hydrolase and metallopeptidase activities of LTA(4) hydrolase also affect the cytotoxicity of the anthrax lethal factor on macrophage cell lines, without interfering with the ability of the lethal factor to enter cells. These results support the proposal that anthrax lethal factor might display in the cytosol of intoxicated cells a peptidase activity similar to that of LTA(4) hydrolase.

引用

页码：687 / 691

页数：5

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