GTT1/StarD7, a novel phosphatidylcholine transfer protein-like highly expressed in gestational trophoblastic turnour: Cloning and characterization

被引:26
作者
Durand, S [1 ]
Angeletti, S [1 ]
Genti-Raimondi, S [1 ]
机构
[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Bioquim Clin, RA-5000 Cordoba, Argentina
关键词
D O I
10.1016/S0143-4004(03)00214-5
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We report the cDNA cloning and characterization of GTT1/StarD7, a novel gestational trophoblastic turnout gene, initially identified by its up-regulated expression in the choriocarcinoma JEG-3 cell line with respect to their nonmalignant counterpart complete hydatidiform mole and normal trophoblastic tissue. Using the differential display fragment as a probe we screened placenta and HeLa cDNA libraries and isolated a clone carrying a 3315 bp insert (accession number AF270647). This cDNA encodes a protein of 295 amino acid residues with a molecular weight of approximately 34.7 kDa and a pI of 5.79. Computer-mediated homology search revealed that the deduced amino acid sequence had similarity to phosphatidylcholine transfer protein (PCTP) with a conserved StAR-related lipid transfer (START) domain extending between the amino acids 66 to 250. The GTT1 gene contains at least 9 exons spread nearly 30 kb on chromosome 2p12-2p11.2. Northern blot assays of total RNA derived from normal early placenta (NEP), complete hydatidiform mole (CHM) and JEG-3 cell line revealed a 3.5 kb mRNA expressed exclusively in the JEG-3 cell line. However, semiquantitative RT-PCR analysis performed with the same RNA samples demonstrated GTT1 expression throughout all of them with the highest level in JEG-3 cell line. Examination of GTT1 mRNA expression by semiquantitative RT-PCR assays in a series of tumour cell lines indicated wide-spread GTT1 expression with predominance in both choriocarcinoma JEG-3 and JAR cells, colorectal adenocarcinoma HT29 and hepatocellular carcinoma HepG2 cells. In conclusion, the highly GTT1 expression profile in JEG-3 and JAR cell lines and its lipid binding domain suggest that GTT1 may play an important role in the phospholipid-mediated signalling of trophoblastic tumour cellular events. (C) 2003 Elsevier Ltd. All rights reserved.
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页码:37 / 44
页数:8
相关论文
共 47 条
  • [1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [2] Phosphatidylcholine metabolism in nuclei of phorbol ester-activated LA-N-1 neuroblastoma cells
    Antony, P
    Kanfer, JN
    Freysz, L
    [J]. NEUROCHEMICAL RESEARCH, 2000, 25 (08) : 1073 - 1082
  • [3] Association of IGF2 and H19 imprinting with choriocarcinoma development
    Arima, T
    Matsuda, T
    Takagi, N
    Wake, N
    [J]. CANCER GENETICS AND CYTOGENETICS, 1997, 93 (01) : 39 - 47
  • [4] BATZER MA, 1992, BIOTECHNIQUES, V12, P370
  • [5] IDENTIFICATION OF DIFFERENTIALLY EXPRESSED MESSENGER-RNA SPECIES BY AN IMPROVED DISPLAY TECHNIQUE (DDRT-PCR)
    BAUER, D
    MULLER, H
    REICH, J
    RIEDEL, H
    AHRENKIEL, V
    WARTHOE, P
    STRAUSS, M
    [J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (18) : 4272 - 4280
  • [6] Bauer M., 1997, General and Diagnostic Pathology, V143, P185
  • [7] INOSITOL PHOSPHATES AND CELL SIGNALING
    BERRIDGE, MJ
    IRVINE, RF
    [J]. NATURE, 1989, 341 (6239) : 197 - 205
  • [8] BOCCO JL, 1989, BIOCHEM INT, V18, P999
  • [9] p21WAF1/CIP1 expression in gestational trophoblastic disease:: correlation with clinicopathological parameters, and Ki67 and p53 gene expression
    Cheung, ANY
    Shen, DH
    Khoo, US
    Wong, LC
    Ngan, HYS
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 1998, 51 (02) : 159 - 162
  • [10] Chilosi M, 1998, LAB INVEST, V78, P269