Diagnosis and follow-up of Whipple's disease by amplification of the 16S rRNA gene of Tropheryma whippelii

被引：28

作者：

Pron, B

Poyart, C

Abachin, E

Fest, T

Belanger, C

Bonnet, C

Capelle, P

Bretagne, JF

Fabianek, A

Girard, L

Hagège, H

Berche, P

机构：

[1] Hop Necker Enfants Malad, Microbiol Serv, F-75743 Paris 12, France

[2] Hop Univ Jean Minjoz, Serv Med Interne, F-25030 Besancon, France

[3] Hop Necker Enfants Malad, Serv Hematol, F-75743 Paris 15, France

[4] Hop Cochin, Serv Hepatol Gastroenterol, F-75014 Paris, France

[5] Clin Bois Verrieres, F-92160 Antony, France

[6] CHRU Pontchaillou, Serv Hepatol Gastroenterol, F-35033 Rennes, France

[7] Ctr Hosp Denain, F-59723 Denain, France

[8] Hop Sud, F-35056 Rennes 2, France

[9] Ctr Hosp Intercommunal, Serv Med Interne & Hepatogastroenterol, F-94010 Creteil, France

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 1999年 / 18卷 / 01期

关键词：

D O I：

10.1007/s100960050228

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Amplification of the 16S rRNA gene of Tropheryma whippelii was performed in eight patients with Whipple's disease and 34 control patients to confirm a diagnosis of Whipple's disease and to monitor the course of disease. Polymerase chain reaction (PCR) tests were positive before treatment in 13 of 15 tissue samples from Whipple's disease patients (gut 8/8: lymph nodes 2/2; bone marrow 1/2; peripheral blood 2/3), in contrast to none of 54 tissue samples from controls. PCR tests converted to negative within 4-6 months in six of the Whipple's disease patients undergoing therapy. These results show that PCR is a reliable and useful tool for diagnosis of Whipple's disease and for monitoring bacterial elimination during antibiotic therapy.

引用

页码：62 / 65

页数：4

共 15 条

[1] Detection of a clonal BCL2 gene rearrangement in tissues from a patient with Whipple disease [J].