The structure of an open form of an E. coli mechanosensitive channel at 3.45 Å resolution

被引：166

作者：

Wang, Wenjian ^{[1
]}

Black, Susan S. ^{[2
]}

Edwards, Michelle D. ^{[2
]}

Miller, Samantha ^{[2
]}

Morrison, Emma L. ^{[2
]}

Bartlett, Wendy ^{[2
]}

Dong, Changjiang ^{[1
]}

Naismith, James H. ^{[1
]}

Booth, Ian R. ^{[2
]}

机构：

[1] Univ St Andrews, Ctr Biomol Sci, St Andrews KY16 9ST, Fife, Scotland

[2] Univ Aberdeen, Sch Med Sci, Inst Med Sci, Aberdeen AB25 2ZD, Scotland

来源：

SCIENCE | 2008年 / 321卷 / 5893期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1126/science.1159262

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

How ion channels are gated to regulate ion flux in and out of cells is the subject of intense interest. The Escherichia coli mechanosensitive channel, MscS, opens to allow rapid ion efflux, relieving the turgor pressure that would otherwise destroy the cell. We present a 3.45 angstrom-resolution structure for the MscS channel in an open conformation. This structure has a pore diameter of similar to 13 angstroms created by substantial rotational rearrangement of the three transmembrane helices. The structure suggests a molecular mechanism that underlies MscS gating and its decay of conductivity during prolonged activation. Support for this mechanism is provided by single-channel analysis of mutants with altered gating characteristics.

引用

页码：1179 / 1183

页数：5

共 36 条

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