Culture and spray-drying of Tsukamurella paurometabola C-924:: stability of formulated powders

被引：11

作者：

Hernandez, Armando

Weekers, Frederic

Mena, Jesus

Pimentel, Eulogio

Zamora, Jesus

Borroto, Carlos

Thonart, Philippe

机构：

[1] Ctr Genet Engn & Biotechnol, Camaguey 70100, Cuba

[2] Artechno SA, Gembloux, Belgium

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 11期

关键词：

formulation; microorganism; nematocidal; powders; spray-drying; stability;

D O I：

10.1007/s10529-007-9436-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nematocidal agent, Tsukamurella paurometabola C-924, was cultured in a 300 l bioreactor. Spray-dried formulations of this microorganism were prepared using sucrose. At an outlet temperature 62 degrees C, survival rates between 12 and 85% were reached with sucrose up to 10% (w/w). The stability study of the powders showed that the best storage condition was at 4 degrees C under vacuum. A new method for the calculation of cell death order for bacteria stored at low temperatures was developed. Powders stored under vacuum showed an Arrhenius behavior in relation to cell death kinetics.

引用

页码：1723 / 1728

页数：6

共 13 条

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