An approach to As(III) and As(V) bioavailability studies with Caco-2 cells

被引:31
作者
Laparra, JM
Vélez, D
Barberá, R
Montoro, R
Farré, R
机构
[1] CSIC, Inst Agroquim & Tecnol Alimentos, Burjassot 46100, Spain
[2] Univ Valencia, Fac Pharm, E-46100 Burjassot, Spain
关键词
arsenite; arsenate; Caco-2; cells; uptake; transport; bioavailability;
D O I
10.1016/j.tiv.2005.05.007
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Foods and drinking water are the main sources of human exposure to inorganic arsenic [As(III) and As(V)]. After oral ingestion, the intestinal epithelium is the first barrier to absorption of these species. A human intestinal cell line (Caco-2) was used to evaluate cell retention and transport of As(III) (15.6-156.0 mu M) and/or As(V) (15.4-170.6 mu M). Cell monolayer integrity, cell viability, membrane damage and effects on cell metabolism were evaluated. Only the highest concentrations assayed [As(111): 156.0 mu M; As(V): 170.6 mu M] produced a cytotoxic effect with different cellular targets: As(III) altered the permeability of tight junctions, and As(V) caused uncoupling of the respiratory chain. Retention and transport of As(111) was more efficient than that of As(V). After 4 h of exposure to As(III) or As(V), monolayer retention percentages varied between 0.87-2.28% and 0.14-0.39%, respectively. Transepithelial transport was greater for As(III) (5.82-7.71%) than for As(V) (not detectable-1.55%). The addition of As(III) and As(V) jointly produced a transport rate similar to that observed when they were added independently. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1071 / 1078
页数:8
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