Multiple regions within the coxsackievirus and adenovirus receptor cytoplasmic domain are required for basolateral sorting

被引：55

作者：

Cohen, CJ ^{[1
]}

Graetz, J ^{[1
]}

Ohman, T ^{[1
]}

Bergelson, JM ^{[1
]}

机构：

[1] Childrens Hosp Philadelphia, Div Immunol & Infect Dis, Philadelphia, PA 19104 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 27期

关键词：

D O I：

10.1074/jbc.M009531200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coxsackievirus and adenovirus receptor (CAR) mediates attachment and infection by coxsackie B viruses and many adenoviruses. In human airway epithelia, as well as in transfected Madin-Darby canine kidney cells, CAR is expressed exclusively on the basolateral surface. Variants of CAR that lack the cytoplasmic domain or are attached to the cell membrane by a glycosylphosphatidylinositol anchor are expressed on both the apical and basolateral surfaces, We have examined the localization of CAR variants with progressive truncations of the cytoplasmic domain, as well as with mutations that ablate a potential PDZ (PSD95/dlg/ZO-1) interaction motif and a putative tyrosine-based sorting signal. In addition, we have examined the targeting of two murine CAR isoforms, with different C-terminal sequences. The results suggest that multiple regions within the CAR cytoplasmic domain contain information that is necessary for basolateral targeting.

引用

页码：25392 / 25398

页数：7

共 33 条

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