Persistent chlamydial envelope antigens in antibiotic-exposed infected cells trigger neutrophil chemotaxis

被引:40
作者
Wyrick, PB
Knight, ST
Paul, TR
Rank, RG
Barbier, CS
机构
[1] Univ N Carolina, Sch Med, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA
[2] Univ Arkansas Med Sci, Dept Immunol & Microbiol, Little Rock, AR 72205 USA
关键词
D O I
10.1086/314676
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
An in vitro coculture model system was used to explore conditions that trigger neutrophil chemotaxis to Chlamydia trachomatis infected human epithelial cells (HEC-1B), Polarized HEC-1B monolayers growing on extracellular matrix (ECM) were infected with C. trachomatis serovar E, By 36 h, coincident with the secretion of chlamydial lipopolysaccharide and major outer membrane protein to the surfaces of infected cells, human polymorphonuclear neutrophils (PMNL) loaded with azithromycin migrated through the ECM and infiltrated the HEC-1B monolayer. Bioreactive azithromycin was delivered by the chemotactic PMNL to infected epithelial cells in concentrations sufficient to kill intracellular chlamydiae. However, residual chlamydial envelopes persisted for 4 weeks, and PMNL chemotaxis was triggered to epithelial cells containing residual envelopes. Infected endometrial cells demonstrated up-regulation of ENA-78 and GCP-2 chemokine mRNA. Thus, despite appropriate antimicrobial therapy, residual chlamydial envelope antigens may persist in infected tissues of culture-negative women and provide one source for sustained inflammation.
引用
收藏
页码:954 / 966
页数:13
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