Salivary Proteins Associated with Periodontitis in Patients with Type 2 Diabetes Mellitus

被引:38
作者
Chan, Hang Haw [1 ]
Rahim, Zubaidah H. A. [1 ]
Jessie, Kala [1 ]
Hashim, Onn H. [2 ,3 ]
Taiyeb-Ali, Tara B. [4 ]
机构
[1] Univ Malaya, Dept Oral Biol, Fac Dent, Kuala Lumpur 50603, Malaysia
[2] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur 50603, Malaysia
[3] Univ Malaya, Fac Med, Ctr Prote Res, Kuala Lumpur 50603, Malaysia
[4] Univ Malaya, Fac Dent, Dept Oral Pathol & Oral Med & Periodontol, Kuala Lumpur 50603, Malaysia
关键词
periodontitis; type 2 diabetes mellitus; salivary protein; 2-DE; INTERLEUKIN-1 RECEPTOR ANTAGONIST; CARBONIC-ANHYDRASE-VI; COMPARATIVE PROTEOMIC ANALYSIS; J-CHAIN; WHOLE SALIVA; DISEASE; IDENTIFICATION; INFLAMMATION; POPULATION; EXPRESSION;
D O I
10.3390/ijms13044642
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
The objective of this study was to investigate the salivary proteins that are associated with periodontitis in patients with Type 2 diabetes mellitus (T2DM). Volunteers for the study were patients from the Diabetic Unit, University of Malaya Medical Centre, whose periodontal status was determined. The diabetic volunteers were divided into two groups, i.e., patients with periodontitis and those who were periodontally healthy. Saliva samples were collected and treated with 10% TCA/acetone/20 mM DTT to precipitate the proteins, which were then separated using two-dimensional polyacrylamide gel electrophoresis. Gel images were scanned using the GS-800 (TM) Calibrated Densitometer. The protein spots were analyzed and expressed in percentage volumes. The percentage volume of each protein spot was subjected to Mann-Whitney statistical analysis using SPSS software and false discovery rate correction. When the expression of the salivary proteins was compared between the T2DM patients with periodontitis with those who were periodontally healthy, seven proteins, including polymeric immunoglobulin receptor, plastin-2, actin related protein 3, leukocyte elastase inhibitor, carbonic anhydrases 6, immunoglobulin J and interleukin-1 receptor antagonist, were found to be differentially expressed (p < 0.01304). This implies that the proteins may have the potential to be used as biomarkers for the prediction of T2DM patients who may be prone to periodontitis.
引用
收藏
页码:4642 / 4654
页数:13
相关论文
共 51 条
[1]
An Autoinflammatory Disease with Deficiency of the Interleukin-1-Receptor Antagonist [J].
Aksentijevich, Ivona ;
Masters, Seth L. ;
Ferguson, Polly J. ;
Dancey, Paul ;
Frenkel, Joost ;
van Royen-Kerkhoff, Annet ;
Laxer, Ron ;
Tedgard, Ulf ;
Cowen, Edward W. ;
Pham, Tuyet-Hang ;
Booty, Matthew ;
Estes, Jacob D. ;
Sandler, Netanya G. ;
Plass, Nicole ;
Stone, Deborah L. ;
Turner, Maria L. ;
Hill, Suvimol ;
Butman, John A. ;
Schneider, Rayfel ;
Babyn, Paul ;
El-Shanti, Hatem I. ;
Pope, Elena ;
Barron, Karyl ;
Bing, Xinyu ;
Laurence, Arian ;
Lee, Chyi-Chia R. ;
Chapelle, Dawn ;
Clarke, Gillian I. ;
Ohson, Kamal ;
Nicholson, Marc ;
Gadina, Massimo ;
Yang, Barbara ;
Korman, Benjamin D. ;
Gregersen, Peter K. ;
van Hagen, P. Martin ;
Hak, A. Elisabeth ;
Huizing, Marjan ;
Rahman, Proton ;
Douek, Daniel C. ;
Remmers, Elaine F. ;
Kastner, Daniel L. ;
Goldbach-Mansky, Raphaela .
NEW ENGLAND JOURNAL OF MEDICINE, 2009, 360 (23) :2426-2437
[2]
[Anonymous], DIAB ATL
[3]
Biological role of interleukin 1 receptor antagonist isoforms [J].
Arend, WP ;
Guthridge, CJ .
ANNALS OF THE RHEUMATIC DISEASES, 2000, 59 :60-64
[4]
Asano Masatake, 2011, J Oral Sci, V53, P147
[5]
CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[6]
Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MSE (Gingival Exudatome) [J].
Bostanci, N. ;
Heywood, W. ;
Mills, K. ;
Parkar, M. ;
Nibali, L. ;
Donos, N. .
JOURNAL OF PROTEOME RESEARCH, 2010, 9 (05) :2191-2199
[7]
Activation of Arp2/3 complex: Addition of the first subunit of the new filament by a WASP protein triggers rapid ATP hydrolysis on Arp2 [J].
Dayel, MJ ;
Mullins, RD .
PLOS BIOLOGY, 2004, 2 (04) :476-485
[8]
Arp2/3 complex requires hydrolyzable ATP for nucleation of new actin filaments [J].
Dayel, MJ ;
Holleran, EA ;
Mullins, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (26) :14871-14876
[9]
Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein [J].
Gabay, C ;
Smith, MF ;
Eidlen, D ;
Arend, WP .
JOURNAL OF CLINICAL INVESTIGATION, 1997, 99 (12) :2930-2940
[10]
Comparative proteomic analysis of whole saliva from chronic periodontitis patients [J].
Goncalves, Lorena Da Ros ;
Soares, Marcia Regina ;
Nogueira, Fabio C. S. ;
Garcia, Carlos ;
Camisasca, Danielle Resende ;
Domont, Gilberto ;
Feitosa, Alfredo C. R. ;
Pereira, Denise de Abreu ;
Zingali, Russolina B. ;
Alves, Gilda .
JOURNAL OF PROTEOMICS, 2010, 73 (07) :1334-1341