Immunosensor for detection of Legionella pneumophila based on imaging ellipsometry

被引：14

作者：

Bae, YM ^{[1
]}

Oh, BK ^{[1
]}

Lee, WC ^{[1
]}

Lee, WH ^{[1
]}

Choi, JW ^{[1
]}

机构：

[1] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2004年 / 24卷 / 1-2期

关键词：

immunosensor; imaging ellipsometry; Legionella pneumophila; self-assembly;

D O I：

10.1016/j.msec.2003.09.049

中图分类号：

T [工业技术];

学科分类号：

08 [工学];

摘要：

An immunosensor based on imaging ellipsometry as detection method was developed for the detection of Legionella pneumophila. Sequential depositions of 11-mercaptoundecanoic acid (11-MUA), protein G, and the monoclonal antibody against L. pneumophila (Mab) on gold surface were confirmed via surface plasmon resonance (SPR). Also, the cross-sensitivity of the Mab against several pathogens including Escherichia coli O157:H7, Salmonella spp. and Yersinia spp. was investigated using the SPR. The immunosensor for detection of L. pneumophila was fabricated by injecting protein G solution using the micro-arrayer and immobilizing the Mab on it. Imaging ellipsometry was used for detection of binding of L. pneumophila to the Mab layer. Ellipsometric images of the protein spots on the substrate according to binding of L. pneumophila with various concentrations were acquired, and the mean optical intensities of them were calculated using the image process software. The low detection limit was about 10(3) CFU/ml. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：61 / 64

页数：4

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