The bacterial toxin ReIE induces specific mRNA cleavage in the A site of the eukaryote ribosome

被引：36

作者：

Andreev, Dmitri ^{[1
]}

Hauryliuk, Vasili ^{[2
]}

Terenin, Ilya ^{[1
]}

Dmitriev, Sergey ^{[1
]}

Ehrenberg, Mans ^{[2
]}

Shatsky, Ivan ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Belozersky Inst Physico Chem Biol, Moscow 11999, Russia

[2] Uppsala Univ, BMC, Dept Cell & Mol Biol, S-75124 Uppsala, Sweden

来源：

RNA | 2008年 / 14卷 / 02期

关键词：

bacterial toxin ReIE; eukaryotic ribosomes; mRNA cleavage; initiation complex assembly; toeprinting; ReIE printing;

D O I：

10.1261/rna.693208

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ReIE/ReIB is a well-characterized toxin-anti-toxin pair involved in nutritional stress responses in Bacteria and Archae. ReIE lacks any eukaryote homolog, but we demonstrate here that it efficiently and specifically cleaves mRNA in the A site of the eukaryote ribosome. The cleavage mechanism is similar to that in bacteria, showing the feasibility of A-site cleavage of mRNA for regulatory purposes also in eukaryotes. ReIE cleavage in the A-site codon of a stalled eukaryote ribosome is precise and easily monitored, making "ReIE printing" a useful complement to toeprinting to determine the exact mRNA location on the eukaryote ribosome and to probe the occupancy of its A site.

引用

页码：233 / 239

页数：7

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