Evidences for involvement of cyclooxygenase-2 in proliferation of two gastrointestinal cancer cell lines

被引:108
作者
Tsuji, S
Kawano, S
Sawaoka, H
Takei, Y
Kobayashi, I
Nagano, K
Fusamoto, H
Kamada, T
机构
[1] First Department of Medicine, Osaka University, School of Medicine, Suita, 565
来源
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1996年 / 55卷 / 03期
关键词
D O I
10.1016/S0952-3278(96)90095-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cyclooxygenase (COX) consists of two isozymes, COX-1 and COX-2. The roles of these isozymes in the gastrointestinal tract are unknown. We investigated messenger RNA expression of the COX-1 and COX-2 genes in the gastrointestinal cancer cell lines MKN28, MKN45, KATO III CACO-2, DLD-1 and LoVo. These cell lines expressed comparable levels of COX-1 mRNA, although their expression of COX-2 varied. Therefore, we studied the effects of NS-398 and indomethacin, specific and non-specific inhibitors for COX-2, on proliferation of the cell lines. Both of the inhibitors suppressed proliferation of the two cell lines that highly expressed COX-2 (MKN45 and CACO-2). However, these inhibitors exerted minimal effects on proliferation of the other cell lines, which expressed significantly lower levels of COX-2. Therefore, it was proposed that COX-2 participates in proliferation of cancer cells because of over expression of the COX-2 gene.
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页码:179 / 183
页数:5
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