The Influence of Copolymer Composition on PLGA/nHA Scaffolds' Cytotoxicity and In Vitro Degradation

被引：49

作者：

Diaz, Esperanza ^{[1
,2
]}

Puerto, Igor ^{[1
]}

Ribeiro, Silvie ^{[3
]}

Lanceros-Mendez, Senentxu ^{[2
,3
,4
]}

Manuel Barandiarian, Jose ^{[2
]}

机构：

[1] Univ Pais Vasco UPV EHU, Dept Ingn Minera Met & Ciencia Mat, Portugalete 48920, Spain

[2] BCMaterials, Parque Cient & Tecnol Bizkaia, Derio 48160, Spain

[3] Univ Minho, Ctr Dept Fis, P-4710057 Braga, Portugal

[4] Basque Fdn Sci, Ikerbasque, Bilbao 48013, Spain

来源：

NANOMATERIALS | 2017年 / 7卷 / 07期

关键词：

scaffolds; PLGA; nHA; cytotoxicity; in vitro degradation; HYDROLYTIC DEGRADATION; TISSUE; BIOMATERIALS;

D O I：

10.3390/nano7070173

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

The influence of copolymer composition on Poly(Lactide-co-Glycolide)/nanohydroxyapatite (PLGA/nHA) composite scaffolds is studied in the context of bone tissue engineering and regenerative medicine. The composite scaffolds are fabricated by thermally-induced phase separation and the effect of bioactive nanoparticles on their in vitro degradation in phosphate-buffered solution at 37 degrees C is analyzed over eight weeks. The indirect cytotoxicity evaluation of the samples followed an adaptation of the ISO 10993-5 standard test method. Based on the measurement of their molecular weight, molar mass, pH, water absorption and dimensions, the porous scaffolds of PLGA with a lower lactide/glycolide (LA/GA) molar ratio degraded faster due to their higher hydrophilicity. All of the samples without and with HA are not cytotoxic, demonstrating their potential for tissue engineering applications.

引用

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