Mitochondrial import of a yeast cytoplasmic tRNA(Lys): Possible roles of aminoacylation and modified nucleosides in subcellular partitioning

被引：24

作者：

Entelis, NS ^{[1
]}

Krasheninnikov, IA ^{[1
]}

Martin, RP ^{[1
]}

Tarassov, IA ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV, FAC BIOL, DIV MOLEC BIOL, MOSCOW 119899, RUSSIA

来源：

FEBS LETTERS | 1996年 / 384卷 / 01期

关键词：

mitochondrial import tRNA(CUU)(Lys); aminoacylation; (Saccharomyces cerevisiae);

D O I：

10.1016/0014-5793(96)00259-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The yeast tRNA(CUU)(Lys) is transcribed from a nuclear gene and then unequally redistributed between the cytosol (97-98%) and mitochondria (2-3%). We have optimized the conditions for its specific import into isolated mitochondria. However, only a minor fraction (about 0.5%) of the added tRNA was translocated into the organelles, An in vitro transcript, once aminoacylated, appeared to be a better import substrate than the natural tRNA which carries modified nucleosides. The tRNA is translocated across mitochondrial membranes in its aminoacylated form and remains relatively stable inside the organelle, Possible roles of aminoacylation, tRNA-protein interactions and nucleoside modification in subcellular partitioning of the tRNA are discussed.

引用

页码：38 / 42

页数：5

共 24 条

[1] STRUCTURE AND EVOLUTION OF A GROUP OF RELATED AMINOACYL-TRANSFER RNA-SYNTHETASES [J].

GATTI, DL ;

TZAGOLOFF, A .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 218 (03) :557-568

[2] TRANSFER-RNAS ARE IMPORTED INTO MITOCHONDRIA OF TRYPANOSOMA-BRUCEI INDEPENDENTLY OF THEIR GENOMIC CONTEXT AND GENETIC-ORIGIN [J].

HAUSER, R ;

SCHNEIDER, A .

EMBO JOURNAL, 1995, 14 (17) :4212-4220

[3] SUBCELLULAR PARTITIONING OF MRP RNA ASSESSED BY ULTRASTRUCTURAL AND BIOCHEMICAL-ANALYSIS [J].

LI, K ;

SMAGULA, CS ;

PARSONS, WJ ;

RICHARDSON, JA ;

GONZALEZ, M ;

HAGLER, HK ;

WILLIAMS, RS .

JOURNAL OF CELL BIOLOGY, 1994, 124 (06) :871-882

[4] SELECTIVE IMPORT OF NUCLEAR-ENCODED TRANSFER-RNAS INTO MITOCHONDRIA OF THE PROTOZOAN LEISHMANIA-TARENTOLAE [J].

LYE, LF ;

CHEN, DHT ;

SUYAMA, Y .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 58 (02) :233-245

[5] IMPORT OF NUCLEAR DEOXYRIBONUCLEIC-ACID CODED LYSINE-ACCEPTING TRANSFER RIBONUCLEIC-ACID (ANTICODON C-U-U) INTO YEAST MITOCHONDRIA [J].

MARTIN, RP ;

SCHNELLER, JM ;

STAHL, AJC ;

DIRHEIMER, G .

BIOCHEMISTRY, 1979, 18 (21) :4600-4605

[6] STUDY OF YEAST MITOCHONDRIAL TRANSFER-RNAS BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS - CHARACTERIZATION OF ISOACCEPTING SPECIES AND SEARCH FOR IMPORTED CYTOPLASMIC TRANSFER-RNAS [J].

MARTIN, RP ;

SCHNELLER, JM ;

STAHL, AJC ;

DIRHEIMER, G .

NUCLEIC ACIDS RESEARCH, 1977, 4 (10) :3497-3510

[7]

MOCZKO M, 1994, J BIOL CHEM, V269, P9045

[8] SUPRAMOLECULAR ORGANIZATION OF THE MAMMALIAN TRANSLATION SYSTEM [J].

NEGRUTSKII, BS ;

STAPULIONIS, R ;

DEUTSCHER, MP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (03) :964-968

[9] RELAXATION OF A TRANSFER-RNA SPECIFICITY BY REMOVAL OF MODIFIED NUCLEOTIDES [J].

PERRET, V ;

GARCIA, A ;

GROSJEAN, H ;

EBEL, JP ;

FLORENTZ, C ;

GIEGE, R .

NATURE, 1990, 344 (6268) :787-789

[10] SEPARATE INFORMATION REQUIRED FOR NUCLEAR AND SUBNUCLEAR LOCALIZATION - ADDITIONAL COMPLEXITY IN LOCALIZING AN ENZYME SHARED BY MITOCHONDRIA AND NUCLEI [J].

ROSE, AM ;

JOYCE, PBM ;

HOPPER, AK ;

MARTIN, NC .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (12) :5652-5658

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