DNA minor groove accessibility in the nucleosome core deduced from specific interactions with DAPI

被引：6

作者：

Caneva, R

Rossetti, L

Savino, M

机构：

[1] IST PASTEUR,FDN CENCI BOLOGNETTI,CNR,CTR STUDIO ACID NUCLEICI,I-00185 ROME,ITALY

[2] UNIV ROMA 1,CNR,CTR STUDIO ACIDI NUCLEICI,I-00185 ROME,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1306卷 / 2-3期

关键词：

nucleosome; 4'; 6-diamidino-2-phenylindole; gene expression; DNA; 4'; 6-DIAMIDINO-2-PHENYLINDOLE DAPI; BINDING MODES; ETHIDIUM;

D O I：

10.1016/0167-4781(96)00015-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Equilibrium binding properties of DAPI for purified nucleosome cores were derived from fluorescence intensity enhancement. Both the affinity and the number of sites of the primary, minor groove binding are preserved with respect to the corresponding isolated DNA, suggesting that the capacity for specific interactions of the minor groove may well be exerted in chromatin.

引用

页码：117 / 121

页数：5

共 25 条

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Bellamy A.R., 1968, METHODS ENZYMOL, V12, P156, DOI [10.1016/0076-6879(67)12125-3, DOI 10.1016/0076-6879(67)12125-3]

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