β2-adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes

We examined the effects of beta 2-adrenergic receptor (beta 2-AR) agonists on monocyte-derived cytokines, interleukin (IL)-18 and IL-12 production in lipopolysaccharide, (LPS)-stimulated monocytes derived from human peripheral blood mononuclear cells (PBMCs), as in vitro model of sepsis. The study found that beta 2-AR agonists inhibited IL-18 and IL-12 production in monocytes, and that AR agonist activity was antagonized by the selective beta 2-AR antagonist, butoxamine. The selective beta 2-AR agonists salbutamol and terbutaline induced a similar inhibitory pattern of IL-18 and IL-12 production. IL-12 production induced by LPS was inhibited by anti-IL-18 Ab, but IL-18 production by LPS was not inhibited by anti-IL-12 Ab, showing that LPS induced IL-18 production without IL-12 production. Therefore, the stimulation of beta 2-AR might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18. (c) 2005 Elsevier B.V. All rights reserved.