Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization

被引：114

作者：

Guo, Z ^{[1
]}

Liu, QH ^{[1
]}

Smith, LM ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706

来源：

NATURE BIOTECHNOLOGY | 1997年 / 15卷 / 04期

关键词：

PCR; specificity; fluorescence; 3-nitropyrrole;

D O I：

10.1038/nbt0497-331

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In order to increase the discrimination of single nucleotide polymorphisms in DNA hybridization, artificial mismatches are inserted into probe oligonucleotides using the base analog 3-nitropyrrole. Differences in thermal stability (Delta T-m) between hybrids formed with normal and single-nucleotide-variant DNA targets are increased by as much as 200% over conventional hybridization, and are strongly dependent upon the spacing between mismatches. The increased specificity is demonstrated by hybridization analysis and allele-specific amplification within the HLA-DRB locus.

引用

收藏

页码：331 / 335

页数：5

相关论文

共 26 条

[1] HLA GENE AMPLIFICATION AND HYBRIDIZATION ANALYSIS OF POLYMORPHISM - HLA MATCHING FOR BONE-MARROW TRANSPLANTATION OF A PATIENT WITH HLA-DEFICIENT SEVERE COMBINED IMMUNODEFICIENCY SYNDROME [J].

BAXTERLOWE, LA ;

HUNTER, JB ;

CASPER, JT ;

GORSKI, J .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (02) :613-618

[2] RAPID HLA-DRB1 GENOTYPING BY NESTED PCR-AMPLIFICATION [J].

BEIN, G ;

GLASER, R ;

KIRCHNER, H .

TISSUE ANTIGENS, 1992, 39 (02) :68-73

[3] SYNTHESIS, STRUCTURE, AND DEOXYRIBONUCLEIC-ACID SEQUENCING WITH A UNIVERSAL NUCLEOSIDE - 1-(2'-DEOXY-BETA-D-RIBOFURANOSYL)-3-NITROPYRROLE [J].

BERGSTROM, DE ;

ZHANG, PM ;

TOMA, PH ;

ANDREWS, PC ;

NICHOLS, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (04) :1201-1209

[4] NOMENCLATURE FOR FACTORS OF THE HLA SYSTEM, 1991 [J].

BODMER, JG ;

MARSH, SGE ;

ALBERT, ED ;

BODMER, WF ;

DUPONT, B ;

ERLICH, HA ;

MACH, B ;

MAYR, WR ;

PARHAM, P ;

SASAZUKI, T ;

SCHREUDER, GMT ;

STROMINGER, JL ;

SVEJGAARD, A ;

TERASAKI, PI .

TISSUE ANTIGENS, 1992, 39 (04) :161-173

[5] PREDICTING DNA DUPLEX STABILITY FROM THE BASE SEQUENCE [J].

BRESLAUER, KJ ;

FRANK, R ;

BLOCKER, H ;

MARKY, LA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) :3746-3750

[6] DETECTION OF SICKLE-CELL BETA-S-GLOBIN ALLELE BY HYBRIDIZATION WITH SYNTHETIC OLIGONUCLEOTIDES [J].

CONNER, BJ ;

REYES, AA ;

MORIN, C ;

ITAKURA, K ;

TEPLITZ, RL ;

WALLACE, RB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :278-282

[7] OPTICAL MELTING OF 128-OCTAMER DNA DUPLEXES - EFFECTS OF BASE-PAIR LOCATION AND NEAREST NEIGHBORS ON THERMAL-STABILITY [J].

DOKTYCZ, MJ ;

MORRIS, MD ;

DORMADY, SJ ;

BEATTIE, KL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) :8439-8445

[8]

*DYN INC, 1989, DYN M 280 TECHN HDB, V6

[9] VERY STABLE MISMATCH DUPLEXES - STRUCTURAL AND THERMODYNAMIC STUDIES ON TANDEM G.A MISMATCHES IN DNA [J].

EBEL, S ;

LANE, AN ;

BROWN, T .

BIOCHEMISTRY, 1992, 31 (48) :12083-12086

[10] DIRECT FLUORESCENCE ANALYSIS OF GENETIC POLYMORPHISMS BY HYBRIDIZATION WITH OLIGONUCLEOTIDE ARRAYS ON GLASS SUPPORTS [J].

GUO, Z ;

GUILFOYLE, RA ;

THIEL, AJ ;

WANG, RF ;

SMITH, LM .

NUCLEIC ACIDS RESEARCH, 1994, 22 (24) :5456-5465