Direct estimation of the recombination frequency between the RB1 gene and two closely linked microsatellites using sperm typing

被引：3

作者：

Girardet, A

Lien, S

Leeflang, EP

Beaufrère, L

Tuffery, S

Munier, F

Arnheim, N

Claustres, M

Pellestor, F

机构：

[1] CHU Montpellier, Inst Biol, Biochim Genet Lab, Montpellier, France

[2] Univ So Calif, Dept Biol Sci, Mol Biol Sect, Los Angeles, CA 90089 USA

[3] Hop Jules Gonin, Lausanne, Switzerland

[4] Inst Genet Humaine, Montpellier, France

来源：

EUROPEAN JOURNAL OF HUMAN GENETICS | 1999年 / 7卷 / 02期

关键词：

sperm typing; recombination frequency; RB1; gene; microsatellites;

D O I：

10.1038/sj.ejhg.5200250

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, single sperm typing has been used for high-resolution recombination analysis between the retinoblastoma gene and two closely linked extragenic microsatellites (D13S284 and D13S1307), The analysis of 1198 single sperm from three donors allowed the determination of recombination fractions between RB1.20 and D13S284 and RB1.20 and D13S1307 of 0.022 and 0.033, respectively. These results show that RB1 gene and the two microsatellites are closely linked, which validates their potential use in indirect genetic diagnosis of retinoblastoma.

引用

页码：239 / 242

页数：4

共 7 条

[1] SINGLE-SPERM TYPING - DETERMINATION OF GENETIC-DISTANCE BETWEEN THE G-GAMMA-GLOBIN AND PARATHYROID-HORMONE LOCI BY USING THE POLYMERASE CHAIN-REACTION AND ALLELE-SPECIFIC OLIGOMERS [J].