Process design for microbial plastic factories: metabolic engineering of polyhydroxyalkanoates

被引：141

作者：

Aldor, AS ^{[1
]}

Keasling, JD ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2003年 / 14卷 / 05期

关键词：

D O I：

10.1016/j.copbio.2003.09.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Implementing several metabolic engineering strategies, either individually or in combination, it is possible to construct microbial plastic factories to produce a variety of polyhydroxyalkanoate (PHA) biopolymers with desirable structures and material properties. Approaches include external substrate manipulation, inhibitor addition, recombinant gene expression, host cell genome manipulation and, most recently, protein engineering of PHA biosynthetic enzymes. In addition, mathematical models and molecular methods can be used to elucidate metabolically engineered systems and to identify targets for performance improvement.

引用

页码：475 / 483

页数：9

共 53 条

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