Rapid genotyping of transforming growth factor β1 gene polymorphisms in a UK Caucasoid control population using the polymerase chain reaction and sequence-specific primers

被引:29
作者
Lympany, PA
Avila, JJ
Mullighan, C
Marshall, S
Welsh, KI
du Bois, RM
机构
[1] Dept Occupat & Environm Med, Interstitial Lung Dis Unit, London SW3 6LR, England
[2] Churchill Hosp, Oxford Transplant Ctr, Tissue Typing Labs, Oxford OX3 7LJ, England
来源
TISSUE ANTIGENS | 1998年 / 52卷 / 06期
关键词
genotyping; haplotyping; PCR-SSP; TGF beta(1);
D O I
10.1111/j.1399-0039.1998.tb03089.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Transforming growth factor (TGF) beta(1) is a growth factor which has multiple functions including the promotion of matrix deposition during wound healing and scar formation. Gene polymorphisms within or close to the 5' region of TGF beta(1) have been identified: three in the upstream region, one in the non-translated region, two in the signal peptide sequence and one in a region of the gene coding for the precursor protein. We have developed a method using 14 polymerase chain reaction-sequence-specific primer reactions to characterise six of these polymorphisms. In the upstream region, both forward and reverse allele-specific primers were used to demonstrate the cis/trans orientation of alleles at adjacent polymorphisms (haplotypes). We report the allele and genotype frequencies of TGF beta(1) genes in two sets of UK Caucasoid control subjects.
引用
收藏
页码:573 / 578
页数:6
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