A subpopulation of bone marrow cells depleted by a novel antibody, anti-Liv8, is useful for cell therapy to repair damaged liver

被引:36
作者
Yamamoto, N
Terai, S
Ohata, S
Watanabe, T
Omori, K
Shinoda, K
Miyamoto, K
Katada, T
Sakaida, I
Nishina, H
Okita, K
机构
[1] Yamaguchi Univ, Sch Med, Dept Mol Sci & Appl Med Gastroenterol & Hepatol, Yamaguchi 7558505, Japan
[2] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Physiol Chem, Bunkyo Ku, Tokyo 1130033, Japan
[3] Yamaguchi Univ, Sch Med, Dept Neuroanat & Neurosci, Yamaguchi 7558505, Japan
[4] Yamaguchi Univ, Sch Med, Dept Mol Sci & Appl Med Kampo Med, Yamaguchi 7558505, Japan
基金
日本学术振兴会;
关键词
bone marrow cell; cell therapy; regenerative medicine; hepatic stem cell; migration; transdifferentiation; mesenchymal stem cell; hematopoietic stem cell; liver regeneration; niche;
D O I
10.1016/j.bbrc.2003.12.044
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We previously reported a new in vivo model named as "GFP/CCl4 model" for monitoring the transdifferentiation of green fluorescent protein (GFP) positive bone marrow cell (BMC) into albumin-positive hepatocyte under the specific "niche" made by CCl4 induced persistent liver damage, but the subpopulation which BMCs transdifferentiate into hepatocytes remains unknown. Here we developed a new monoclonal antibody, anti-Liv8, using mouse E 11.5 fetal liver as an antigen. Anti-Liv8 recognized both hematopoietic progenitor cells in fetal liver at E 11.5 and CD45-positive hematopoietic cells in adult bone marrow. We separated Liv8-positive and Liv8-negative cells and then transplanted these cells into a continuous liver damaged model. At 4 weeks after BMC transplantation, more efficient repopulation and transdifferentiation of BMC into hepatocytes were seen with Liv8-negative cells. These findings suggest that the subpopulation of Liv8-negative cells includes useful cells to perform cell therapy on repair damaged liver. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:1110 / 1118
页数:9
相关论文
共 34 条
  • [1] Cell differentiation - Hepatocytes from nonhepatic adult stem cells
    Alison, MR
    Poulsom, R
    Jeffery, R
    Dhillon, AP
    Quaglia, A
    Jacob, J
    Novelli, M
    Prentice, G
    Williamson, J
    Wright, NA
    [J]. NATURE, 2000, 406 (6793) : 257 - 257
  • [2] Liver repopulation after cell transplantation in mice treated with retrorsine and carbon tetrachloride
    Gu, DQ
    Fu, T
    Nelson, JA
    Superina, RA
    Soriano, HE
    [J]. TRANSPLANTATION, 2002, 73 (11) : 1818 - 1824
  • [3] Transplanted hepatocytes proliferate differently after CCl4 treatment and hepatocyte growth factor infusion
    Gupta, S
    Rajvanshi, P
    Aragona, E
    Lee, CD
    Yerneni, PR
    Burk, RD
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1999, 276 (03): : G629 - G638
  • [4] Hatch Heather M., 2002, Cloning and Stem Cells, V4, P339, DOI 10.1089/153623002321025014
  • [5] Plasticity of marrow-derived stem cells
    Herzog, EL
    Chai, L
    Krause, DS
    [J]. BLOOD, 2003, 102 (10) : 3483 - 3493
  • [6] Pluripotency of mesenchymal stem cells derived from adult marrow
    Jiang, Yuehua
    Jahagirdar, Balkrishna N.
    Reinhardt, R. Lee
    Schwartz, Robert E.
    Keene, C. Dirk
    Ortiz-Gonzalez, Xilma R.
    Reyes, Morayma
    Lenvik, Todd
    Lund, Troy
    Blackstad, Mark
    Du, Jingbo
    Aldrich, Sara
    Lisberg, Aaron
    Low, Walter C.
    Lergaespada, David A.
    Verfaillie, Catherine M.
    [J]. Nature, 2002, 418 (6893) : 41 - 49
  • [7] Little evidence of bone marrow-derived hepatocytes in the replacement of injured liver
    Kanazawa, Y
    Verma, IM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 : 11850 - 11853
  • [8] Cytokine regulation of liver development
    Kinoshita, T
    Miyajima, A
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2002, 1592 (03): : 303 - 312
  • [9] HGF, SDF-1, and MMP-9 are involved in stress-induced human CD34+ stem cell recruitment to the liver
    Kollet, O
    Shivtiel, S
    Chen, YQ
    Suriawinata, J
    Thung, SN
    Dabeva, MD
    Kahn, J
    Spiegel, A
    Dar, A
    Samira, S
    Goichberg, P
    Kalinkovich, A
    Arenzana-Seisdedos, F
    Nagler, A
    Hardan, Z
    Revel, M
    Shafritz, DA
    Lapidot, T
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2003, 112 (02) : 160 - 169
  • [10] Hepatocytes and epithelial cells of donor origin in recipients of peripheral-blood stem cells.
    Korbling, M
    Katz, RL
    Khanna, A
    Ruifrok, AC
    Rondon, G
    Albitar, M
    Champlin, RE
    Estrov, Z
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2002, 346 (10) : 738 - 746